In PCR what is the chemical makeup of the primer, is made of DNA or RNA? I can't get a straight simple answer anywhere

2018-01-24 07:00:30

I'm thinking the answer is RNA. Is that right?

Almost always DNA. While RNA is possible, it’s much harder to synthesize (and keep stable) and DNA results in a purely DNA result that won’t need to be replaced with DNA later.

PCR primers are short pieces of single-stranded DNA, usually around

20 nucleotides in length.

You can read more about PCR on Khan Academy or Wikipedia.

  • Almost always DNA. While RNA is possible, it’s much harder to synthesize (and keep stable) and DNA results in a purely DNA result that won’t need to be replaced with DNA later.

    2018-01-24 07:11:15
  • PCR primers are short pieces of single-stranded DNA, usually around

    20 nucleotides in length.

    You can read more about PCR on Khan Academy or Wikipedia.

    2018-01-24 08:12:38